Coding

Part:BBa_K2048001:Design

Designed by: Seray Cicek   Group: iGEM16_Toronto   (2016-10-13)


Long GolS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 418
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our main goal was to generate a circuit that allows production of GolS transcriptional factor and activate production of LacZ. This is achieved by placing golB before the LacZ region


Source

Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

References